Jack Riewoldt is resting with a minor quad complaint and will miss Richmond's second NAB Challenge match.
But the key forward is expected to be fit to play the following practice matches and against Gold Coast in round one.
Riewoldt, midfielder Daniel Jackson, defender Dylan Grimes and ruckman Ivan Maric all missed the club's first pre-season game and will not play against Collingwood in Wangaratta on Saturday, but the club remains confident they will all be fit for the first home-and-away match. ''Jack has had a bit of a quad niggle, just some tightness, which we don't think is serious but we want to give him another week,'' football manager Dan Richardson said.
Richmond will play an intra-club match next week and then a practice match against Essendon on March 7. Players needing more match practice could also take part in a VFL practice match in the final week before their season starts on March 15.
Running defender and midfielder Reece Conca will play his first pre-season game on Saturday after hamstring and foot surgery.
Maric is recovering from groin surgery and is back in full training while Grimes is likely to play in the next fortnight.
Small forward Jake King will play in Wangaratta after building up his running following a big-toe injury.
''I had a big foot operation - I hurt my big toe half-way through the season - and I had to get a good clean out of my shoulder done,'' King told the Richmond website.
''The big toe has been the big setback. We don't think about it all that much but it is where you put all your pressure as a footballer through the feet and through your toe, so that has been causing me a bit of drama. But we are on top of that now and I am back into full training with the main group.
''The drama with the toe is with footy you have to run and [the injury] restricts you from running for a couple of months and you have to get your fitness some other way.''
Richardson said that unless a player was injured in the next few weeks the club would have a full list available for round one.
Collingwood star Dane Swan is expected to play on Saturday, as is Alex Fasolo, who has not played since round five last season due to a stress fracture in his foot.
Key defender Nathan Brown and tall utilities Ben Reid and Tyson Goldsack are also part of the 29-man squad, which also includes Caolan Mooney and ruckman Ben Hudson.
Dayne Beams and ex-skipper Nick Maxwell will be rested, while Nathan Freeman (hamstring) and Tim Broomhead (hand) are likely to miss a month.